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. 2022 Mar 19;16(9):1447–1460. doi: 10.1093/ecco-jcc/jjac047

Figure 2.

Figure 2.

Overview of the calprotectin sequence of both A] S100A9 and B] S100A8 dimers. The calprotectin fragments generated by human neutrophil elastase [HNE]-mediated cleavage and selected for ELISA development are depicted by an arrow down [↓], where the neo-epitope selected for ELISA development is depicted by the color grey. Relative peptide abundance of selected HNE-derived calprotectin neo-epitope cleavage fragments; antibody specificity test and biological evaluation of the CPa9-HNE ELISA. C] The relative peptide abundance for the CPa9-HNE sequence was tested using mass spectrometry in samples containing HNE-cleaved calprotectin, full-length calprotectin, or HNE. Furthermore, D] antibody specificity was tested against the selection peptide, elongated peptide, truncated peptide, non-sense peptide, and finally E] biological evaluation of the CPa9-HNE assay was tested in samples containing HNE + calprotectin, calprotectin [Control 1], and HNE [Control 2]. Error bars represent the standard error of the mean [SEM]. ELISA, enzyme-linked immunosorbent assay.