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. 2022 Mar 19;16(9):1447–1460. doi: 10.1093/ecco-jcc/jjac047

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© The Author(s) 2022. Published by Oxford University Press on behalf of European Crohn’s and Colitis Organisation.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Ex vivo cultures of neutrophil granulocytes demonstrating CPa9-HNE biomarker level fold-change compared with inactive neutrophils, reflecting true neutrophil activity. CPa9-HNE biomarker levels after A] 4 h of cultivation with neutrophil agonists B] and 24 h of cultivation; MRP8/14 biomarker measurements after C] 4 h of cultivation with neutrophil agonists D] and 24 h of cultivation. Definition: negative control [only tissue], S. aureus [tissue + Staphylococcus aureus], inactive neutrophils [tissue + neutrophils], neutrophils + S. aureus [tissue + neutrophils + Staphylococcus aureus], neutrophils], neutrophils + PMA [tissue + neutrophils + phorbol myristate acetate], neutrophils + Cal iono [tissue + neutrophils + calcium ionophore]. Error bars represent the standard error of the mean [SEM].