Table 1.

CPa9-HNE ELISA parameters.

Species possibilities	Human
ELISA range [ng/ml] LLMR-ULMR	9–493 ng/mL [adjusted: 36.3–1970 ng/mL]
IC50 [mean, ng/ml]	78.34
Slope [mean]	0.94
Intra-ELISA mean CV%, QC	9% [5.2-15%]
Inter-ELISA mean CV%, QC	4.3% [1.5-9.3%]
Analyte recovery [%], 24 h, 4°C/20°C	91%/95%
Analyte recovery [%], 48 h, 4°C/20°C	114%/138%
Sample recovery [%], freeze/thaw cycles, 3F/T cycles/4F/T cycles	93%/91%
Dilution recovery [%], serum [MRD = 1:4]	96%
Dilution recovery [%], EDTA [MRD = 1:0]	92%
Spiking recovery [%], serum	101%
Spiking recovery [%], EDTA	99%
Interference, biotin recovery [%] [low/high]	98%/95%
Interference, lipaemia recovery [%] [low/high]	100%/97%
Interference, haemoglobin [%] recovery [low/high]	91%/72%

Percentages are reported as mean.

ELISA, enzyme-linked immunosorbent assay; EDTA, ethylene diamine tetraacetic acid; FT, freeze/thaw; LLMR, lower limit of measurement range; ULMR, upper limit of measurement range; IC50, 50% inhibitory concentration; CV%, coefficient of variance %; QC, quality control samples; MRD, maximum [or minimum] recovery diluent.