Figure 7.

MA-5-induced suppression in mitofilin/immt-1 gene mutation against abnormal mitochondrial morphology. immt-1 (tm1730) null mutation ATU3307 worms expressing mitochondria-targeted green fluorescent protein (mtGFP) and nuclear-targeted GFP–LacZ (nucGFP) in body wall muscle cells were treated with or without MA-5 from L4 to adult stage on day 4. (A) Representative images of mitochondrial morphologies are shown (scale bar: 20 µm). White arrows indicate body wall muscle cell nuclei, and white arrowheads indicate abnormally swollen mitochondria. (B) The number of abnormal mitochondria in each muscle cell (n ≥ 35 from 6–8 worms/treatment). Data are shown as box plots to indicate median (central line) and mean (square mark). Different letters indicate significant differences (p ≤ 0.05) using Dunn’s test with all data points including outliers. (C) Observation of the mitochondria using transmission electron microscopy (scale bar: 500 nm). Red arrows indicate abnormal mitochondria and red arrowheads indicate abnormal cristae. WT: wild-type strain ATU3301 as mentioned before; Cont: control treated with 0.1% DMSO; MA-5: 10 μM; Diamond mark: outlier data.