Table 3. Factors associated with FBB18 that are involved in dynein preassembly.
| Proteins | Gene ID | Control Spectral count (unique peptides) |
FBB18-YFP Spectral count (unique peptides) |
|---|---|---|---|
| DNAAFs that are associated with FBB18 | |||
| DNAAF1/DAU1/ODA7 | Cre01.g029150 | - | 11 (6) |
| DNAAF2/ PF13 | Cre09.g411450 | - | 7 (5) |
| DNAAF3/ PF22 | Cre01.g001657 | - | 28 (7) |
| DNAAF4/ DYX1C1/ PF23 | Cre11.g467560 | - | 3 (3) |
| DNAAF5/ HEATR2 | Cre09.g395500 | 44 (21) | 94 (25) |
| DNAAF6/ TWI1/ PIH1D3 | Cre07.g335800 | - | 9 (5) |
| DNAAF10/WDR92 | Cre16.g672600 | - | 3 (3) |
| DNAAF11/MOT47/ LRRC6 | Cre17.g739850 | 1(1) | 5 (4) |
| DNAAF16/FBB18/CFAP298 | Cre16.g688450 | - | 582 (31) |
| DNAAF17/FBB5/ C11orf70/CFAP300 | Cre12.g556300 | 3 (3) | 37 (14) |
| DNAAFs that are not associated with FBB18 | |||
| DNAAF7/ZMYND10 | Cre08.g358751 | 19(7) | 12(6) |
| DNAAF9/Shulin/C20orf194 | Cre11.g467556 | 1(1) | 1(1) |
| DNAAF12/LRRC56/ODA8 | Cre01.g043650 | - | - |
| DNAAF13/SPAG1 | Cre12.g556100 | 1(1) | (1) |
| DNAAF14/MOT48 | Cre10.g467050 | - | - |
| DNAAF18/DAW1/ODA16/WDR69 | Cre04.g216902 | - | - |
| RPAP3 | Cre02.g084900 | - | - |
| Chaperones and co-chaperones | |||
| RuvBL1 | Cre09.g414050 | 9 (5) | 220 (25) |
| RuvBL2 | Cre10.g442700 | 7 (5) | 188 (29) |
| HSP90A | Cre09.g386750 | 17 (9) | 48 (17) |
| HSP90C | Cre12.g514850 | - | 8 (6) |
| HSP70E | Cre16.g677000 | 3 (2) | 8 (3) |
Immunoprecipitates from cells expressing FBB18:YFP-TG or control cells with anti-GFP antibody were analyzed by mass spectrometry. Known dynein preassembly factors, R2TP components and chaperones were searched in the data set and their abundances are presented. Please note that the homologues of DNAAF8 and 15 are not present in the Chlamydomonas proteome. RPAP3 forms a R2TP complex with DNAAF14 and RuvBL1/2 and is thus being considered as a possible DNAAF (PMID: 30428028).