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. 2022 Aug 23;23(17):9524. doi: 10.3390/ijms23179524

Figure 3.

Figure 3

WDR45-mutant fibroblasts exhibit altered autophagy. Western blot analysis of total protein extract from fibroblasts from: (a) WDR45 mutation carrier (P1-Patient 1) and three healthy controls (Ctrl1, Ctrl2, Ctrl3) with antibody against the autophagosome marker LC3-II (membrane-bound form), and β-actin (loading control) under basal conditions, (b) and upon treatment with 10 nM of Bafilomycin A1 for 6 h. (c) Patient 1’s fibroblasts (P1) and three healthy controls (Ctrl1, Ctrl2, Ctrl3) with antibodies against Beclin 1 and β-actin (loading control). Data analysis was carried out for all Western Blots with Ctrl1 set as 1. The error bars indicate the standard error of the mean of n ≥ 3 independent experiments. Statistical significance was analyzed by Unpaired t-test (ns refers to p ≥ 0.05; ** p < 0.01).