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. 2022 Aug 23;23(17):9524. doi: 10.3390/ijms23179524

Figure 5.

Figure 5

Loss of WDR45 is linked to disrupted iron recycling via ferritinophagy. Western blot analysis of total protein extract from fibroblasts from: WDR45 mutation carriers (P1 and P2) and two healthy controls (Ctrl1 and Ctrl2) with antibody against NCOA4 and β-actin (loading control) under basal conditions (BC) and upon treatment with 5, 100, 500, 1000 and 2500 µM of FAC for 24 h. Data analysis was carried out for all Western blots with Ctrl1 BC and P1 (for the upper panel) and Ctrl2 BC and P2 (for the lower panel) set as 1. The error bars indicate the standard error of the mean of n ≥ 3 independent experiments. One-way ANOVA analyzed statistical significance with post-hoc Turkey test (ns refers to p ≥ 0.05 (not significant); *** p < 0.001 and **** p < 0.0001 refers to significantly increased NCOA4 protein levels in fibroblasts treated with 2500 µM of FAC compared to the same but untreated cells (BC)).