FIG. 1.

Western analysis of AhpC, AhpF, and OxyR in XpHR and the parental strain. X. campestris pv. phaseoli (Xp) and XpHR were grown aerobically to mid-log phase in Silva Buddenhagen (SB) medium at 28°C. Cell lysate preparation, gel electrophoresis, blotting to nitrocellulose membranes, and antibody reactions were performed as previously described (17). Antibody reactions were subsequently detected with an anti-rabbit antibody conjugated to alkaline phosphate. Total protein (50 μg) was loaded into each lane. Western blots were treated with an anti-AhpC (AhpC), an anti-AhpF (AhpF), and an anti-OxyR (OxyR) antibody, respectively.