FIG. 3.

Effects of various mutations in oxyR on levels of AhpC during uninduced and menadione-induced growth. Western analysis of AhpC levels in X. campestris pv. phaseoli oxyR harboring various oxyR genes on an expression vector from a parental strain (pBBRoxyR1), the XpHR mutant (pBBRoxyR5), and the gene with one amino acid changed (pBBRoxyR5G197D and pBBR oxyR5L301R). These cells were grown uninduced (U) in Silva Buddenhagen (SB) medium or induced with 100 μM menadione (I) for 30 min. Total protein (20 μg) was loaded into each lane. Lysate preparation, gel electrophoresis, and antibody reactions were performed as described previously (17) and in the legend to Fig. 1.