Table 5.

Primers used for RT-qPCR.

Gene	Primer Direction	Primer Sequence (5′–3′)	Position	Amplicon Length (bp)	Acc No	Reference
GAPDH	forward	GTC ATC AAC GGG AAG TCC ATC TC	196–218	84	AB038240	von Smolinski et al., 2005 [179]
	reverse	AAC ATA CTC AGC ACC AGC ATC AC	279–257
CDV	forward	GCT CTT GGG TTG CAT GAG TT	954–973	83	AF378705	Puff et al., 2008 [176]
	reverse	GCT GTT TCA CCC ATC TGT TG	1036–1017
IL-1-β	forward	TGT CAG TCA TTG TAG CTT TG	123–142	113	Z70047	Primer-BLAST [181]
	reverse	GCA GAT GAT AGG TTC TTC TT	235–216
IL-2	forward	CCA ACT CTC CAG GAT GCT CAC	196–216	81	D30710	Schwartz et al., 2011 [177]
	reverse	TCT GCT AGA CAT TGA AGG TGT GTA	276–252
IL-4	forward	CTC CAA AGA ACA CAA GCG ATA AGG	239–262	84	AF239917	Schwartz et al., 2011 [177]
	reverse	TGT TGG AGC AGT TGT GTG TAT AGA	322–299
IL-6	forward	TGA TGC CAC TTC AAA TAG TCT ACC A	156–180	89	U12234	Spitzbarth et al., 2011 [180]
	reverse	TCA GTG CAG AGA TTT TGC CGA GGA	244–221
IL-8	forward	TTC GAT GCC AGT GTA TAA AA	130–149	74	D28772	Primer-BLAST [181]
	reverse	GTC AAT CAC TCT CAG TTC TT	203–184
IL-10	forward	ACC ACG ACC CAG ACA TCA AGA A	303–324	120	U33843	Primer-BLAST [181]
	reverse	CCT TGC TCT TAT TCT CAC AGG GAA G	422–398
IL-12	forward	CTC GTT TTG CTG GCG TCT TC	37–56	153	U49100	Primer-BLAST [181]
	reverse	CGC TGA GGT CCA AGT GAT GT	189–170
TNF-α	forward	GGA GCT GAC AGA CAA CCA GCT GA	133–155	91	Z70046	Spitzbarth et al., 2011 [180]
	reverse	GGA AGG GCA CCC TTG GCC CT	223–204
TGF-β	forward	TGG CGC TAC CTC AGC AAC CG	592–611	115	L34956	Spitzbarth et al., 2011 [180]
	reverse	AGC CCT CGA CTT CCC CTC CA	706–687
IFN-γ	forward	AGC ATG GAT ACC ATC AAG GAA GA	223–245	104	S41201	Schwartz et al., 2011 [177]
	reverse	AGA TCG TTC ACA GGA ATT TGA ATC A	326–302

bp = base pairs; Acc No = GenBank accession number; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; CDV = canine distemper virus; IL = interleukin; TNF-α = tumor necrosis factor alpha; TGF-β = transforming growth factor beta; IFN-γ = interferon gamma.