Figure 5.

Lumican-stimulated myogenesis and protein balance mediated by p38 MAPK. (A) C2C12 cells were treated with or without 10 nM lumican for indicated periods. Total cell lysates were subjected to western blotting with relevant antibodies. Quantitative results are shown in the right panels. (B) C2C12 cells were differentiated with 10 nM lumican in the presence or absence of SB203580 for 3 days. Myotubes were stained with the anti-myosin heavy chain antibody. Quantitative results of myotube area per myotube are shown. Each bar represents the mean ± standard error of the mean (SEM). * p < 0.05 vs. lumican-untreated control. NS, not significant; MAPK, mitogen-activated protein kinase.