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. 2022 Sep 4;23(17):10130. doi: 10.3390/ijms231710130

Figure 6.

Figure 6

Histologic analysis and survivability of zebrafish injected with carboxymethyllysine (CML) in the presence or absence of CIGB-258. (A) Visualization of the infiltration of neutrophils by Hematoxylin and Eosin (H&E) staining as shown in top photographs (photo a and b, 400× magnification). The bottom photographs show conversion of the Hematoxylin-stained area into red intensity (photo c and d, 400× magnification). (B) Quantification of the nucleus area from H&E staining using Image J software (http://rsb.info.nih.gov/ij/, accessed on 15 April 2022) as shown in red intensity and survivability of zebrafish at 60 min post-injection. *** p < 0.001 versus CML + PBS.