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. 2022 Sep 5;23(17):10163. doi: 10.3390/ijms231710163

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of trifuhalol A on the serum and tissue factors related to allergic inflammation in HDM-induced AD-like NC/Nga mice. (a) Serum IgE and (b) IL-4 levels were measured using ELISA (n = 5 in each group). Immunohistochemistry was used to identify the expression level and sites of (c) IL-33 and (d) TSLP proteins in the skin (n = 5 in each group) (magnification ×100). Representative data of qPCR analysis of (e) IL-4 and (f) IL-13 mRNA normalized to GAPDH. Each bar represents the mean ± SE (n = 5 in each group). ^# p < 0.05 vs. control group; * p < 0.05 vs. HDM group.