Figure 1.

Sequence analysis of Hcp from Acidovorax citrulli Aac5. (a) Multiple sequence alignment of Hcp amino acid sequences from A. citrulli Aac5, AAC00-1, M6, and A. avenae ATCC19860. The alignment was performed using CLUSTALW2 and visualized in Jalview. (b) Maximum-likelihood phylogeny based on representative Hcp protein sequences. The evolutionary relationship was inferred in MEGA11 using the Maximum likelihood method and the JTT matrix-based model. The tree with the highest log likelihood (−8853.75) is shown. The bootstrap values are shown above the branches. Initial tree(s) for the heuristic search were obtained automatically by applying the Neighbor-Joining and BioNJ algorithms to a matrix of pairwise distances estimated using the JTT model and then selecting the topology with superior log-likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The tree was rooted with the outgroup Hcp2 of RIMD2210633 BAC62370.1, which has relatively low homology with other Hcps.