Figure 2.

Growth ability of Acidovorax citrulli WT Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm in vitro. (a,b) Growth ability of the Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm strains in KB medium starting at 1 × 10⁶ CFU·mL⁻¹ and 1 × 10⁷ CFU·mL⁻¹, respectively. (c) Growth ability of Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm in M9 at an initial concentration of 1 × 10⁶ CFU·mL⁻¹. (d) Growth ability of Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm in M9-Fe³⁺ (12.5 mmol·L⁻¹ Fe³⁺) at an initial concentration of 10⁶ CFU·mL⁻¹. Cell suspensions of each strain grown to a density of OD₆₀₀ of 0.3 were diluted 10-fold and 100-fold in liquid KB, M9, and M9-Fe³⁺ media, and then 200 µL of the suspensions were added to wells of a 100-well plate. Three biological replicates were performed per A. citrulli strain per experiment and the experiment was conducted three times. The plate was incubated at 28 °C with shaking at 220 rpm/min, and the OD₆₀₀ was measured every 2 h for 96 h in a Bioscreen C chamber.