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. 2022 Aug 25;23(17):9632. doi: 10.3390/ijms23179632

Figure 5.

Figure 5

Interspecies competition of Acidovorax citrulli strains (Ac strains for short) Aac5 (WT), Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm with Escherichia coli DH5α-pBBRMCS5, Pseudomonas aeruginosa ATCC27853, Saccharomyces cerevisiae Y2HGold, and Pichia pastoris GS115. (a) Survival of E. coli DH5α-pBBRMCS5 on LB amended with gentamicin (Gm) after 2 h of co-culture with A. citrulli killer strains (Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm) at a killer:prey ratio of 20:1. (b) Survival of Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm strains on KB amended with Amp and Km after 2 h of co-culture with DH5α-pBBRMCS5. (c) Survival of P. aeruginosa ATCC27853 on CFC selective agar after 4 h of co-culture with the killer strains (Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm) at a killer:prey ratio of 30:1. (d) Survival of Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm on KB amended with Amp and Km after 4 h of co-culture with ATCC27853. (e) Survival of S. cerevisiae Y2HGold on YPDA agar amended with Gm after 12 h of co-culture with killer strains (Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm) at a killer:prey ratio of 30:1. (f) Survival of Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm on KB amended with Amp, Km, and AbA after 12 h of co-culture with Y2HGold. (g) Survival of Pichia pastoris GS115 on YPDA medium amended with Gm after 12 h of co-culture with killer strains (Aac5, Δhcp, Δhcpcomp, Δtssm and ΔhcpΔtssm) at a killer:prey ratio of 30:1. (h) Survival of Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm on KB medium amended with Amp, Km, and AbA after 12 h of co-culture with GS115. All strains were cultured to an OD600 = 1.5 and co-cultured in 24-well-plates with ddH2O. After co-culture, the mixtures were ten-fold serially diluted and spotted onto plates with antibiotics or selective media. The survival of both killer and prey cells was quantified by colony count and converted to log10CFU·mL−1. Significant differences were found between different letter substitutes on the column calculated by Duncan’s new multiple range test (p = 0.05).