Figure 6.

Intraspecies competition between Acidovorax citrulli group I strain pslb65-pBBRMCS5 and group II strains Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm. (a) Survival of pslb65-pBBRMCS5 in KB medium amended with Ampicillin (Amp), and Gentamicin (Gm) after 3 h of co-culture with the killer strains (Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm) at a killer:prey ratio of 30:1. (b) Survival of Aac5, Δhcp, Δhcpcomp, Δtssm, and ΔhcpΔtssm in KB amended with Amp and Km after 3 h of co-culture with pslb65-pBBRMCS5. All strains were cultured to OD₆₀₀ = 1.5 and co-cultured in 24-well-plates with ddH₂O. After co-culture, the mixtures were ten-fold serially diluted and spotted onto plates amended with antibiotics. The survival of both killer and prey cells was quantified by colony count and converted to log₁₀CFU·mL⁻¹. Significant differences were found between different letter substitutes on the column calculated by Duncan’s new multiple range test (p = 0.01).