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. 2022 Sep 4;23(17):10119. doi: 10.3390/ijms231710119

Figure 4.

Figure 4

HIF1-mediated phosphorylation of TAZ at Ser89 under hypoxia inbasal A but not luminal breast cancer cells. (A,B) Western blot showing YAP, TAZ, p-YAP (Ser127) and p-TAZ (Ser89) for three luminal (MCF7, T47D and BT474) and three basal A (SUM149PT, HCC1806 and HCC1143) breast cancer cell lines incubated under normoxia (NX) or hypoxia (HX) for 3 or 5 (A) or 10 days (B). Tubulin serves as loading control. (C) Western blot showing the indicated (phospho-)proteins for the indicated luminal and basal A cells seeded at low density (6 × 104 cells/petri dish; subconfluent) or high density (15e4 cells/petri dish; confluent) and exposed for 5 days to normoxia or hypoxia. (D) Western blot analysis of the indicated (phospho-)proteins for SUM149PT exposed for 3 or 5 days to normoxia or hypoxia. (E) Western blot analysis of the indicated (phospho-)proteins for HCC1143 cells treated with TAZ (WWTR1) SMARTpool siRNAs or the indicated control SMARTpool siRNAs and exposed for 5 days to hypoxia. (F) Western blot showing p-TAZ (Ser89) and TAZ in HCC1806 and HCC1143 cells cultured in absence or presence of Verteporfin (5 µM) or BV02 (5 µM) while exposed to normoxia, hypoxia, or DMOG (1 mM) for 3 days. β-actin serves as loading control.