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. 2022 Sep 4;23(17):10119. doi: 10.3390/ijms231710119

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Subcellular localization of phospho-YAP/TAZ upon YAP knockout in HCC1806 cells under hypoxia. (A–C) YAP (A), TAZ (B) and phospho-YAP/TAZ (C) expression and localization analyzed by confocal immunofluorescence microscopy after 5-day incubation under hypoxia in CAS9 expressing HCC1806 cells transduced with YAP- or non-targeting control lentiviral sgRNA constructs. (D) Detection of YAP, TAZ, p-YAP (Ser127), and p-TAZ (Ser89) by Western blot after 5-day incubation under hypoxia in nuclear and cytoplasmic fractions of CAS9 expressing HCC1806 cells transduced with YAP- or non-targeting control lentiviral sgRNA constructs. Tubulin and Lamin A/C serve as loading controls for cytoplasmic and nuclear fraction, respectively (E) Longer exposure of Western blot used for (D).