Table 4.
Characterization of the liposomal formulations.
| Lipid Composition (Molar Ratio) |
Lysin | (Prot/Lip)i (μg/μmol) | (Prot/Lip)f (μg/μmol) | E.E. (%) | Mean Size (nm) | P. I. | Zeta Pot (mV) |
|---|---|---|---|---|---|---|---|
| DPPC:DOPE:CHEMS (4:4:2) |
Pa7 | 16 ± 5 | 5 ± 2 | 33 ± 7 | 151 ± 6 | 0.111 ± 0.011 | −21 ± 2 |
| DPPC:DOPE:CHEMS (4:4:2) |
Pa119 | 10 ± 3 | 3 ± 1 | 32 ±9 | 149 ± 4 | 0.106 ± 0.012 | −22 ± 2 |
| DPPC:DOPE:CHEMS (4:4:2) |
EL | NA | NA | NA | 142 ± 2 | 0.125 ± 0.015 | −22 ± 1 |
Values are presented as average ± standard deviation, based on 6 to 9 independent experiments. NA—not applicable; Prot—protein; Lip—lipid; E.E.—encapsulation efficiency; P.I.—polydispersion index; EL—empty liposomes. Lysin Pa7 initial concentration ranged from 650 to 850 μg/mL. Lysin Pa119 initial concentration ranged from 500 to 750 μg/mL.