Figure 5.

Experimental design and sex determination. (A) Experimental design. In this study, four chicken breeds and five embryonic important time points were selected to explore the expression levels of three muscle-related genes in pectoral muscle and thigh muscle. In addition, the H&E slices were made for the observation of the histomorphology of myofibers. (B) Graphical representation of different sexes. For males, both sides of the gonads were the same size; for females, the left side of the gonads was larger than the right side. (C) Reconfirmation of sexes by gel mapping. For males (M), one band (600 bp) was visible; for females (F), two bands (600 bp and 450 bp) were visible.