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Hemoglobin re-oxidation kinetics after rejuvenation performed at the second (a); eighth (b), and eleventh day (c) for static controls (red squares) and mechanically stimulated (blue circles) samples. The Soret peak in shaken cells always occurs at shorter wavelengths (i.e., is more oxidated) than in static controls. This indicates that Hb re-oxidation is always faster for mechanically stimulated cells, suggesting that the shaken RBCs experience a higher stress condition.
