Figure 9.

Panels (a,b) report, respectively, the percentage of cell lysis in static controls (a) and in mechanically stimulated cells (b) in the presence and absence of DIAM, CARB, GLIB, and PROB. Moreover, in panels (c,d) we compared the ratio of the lysis curves (shown in Figure 2a,b) for CTR/PROB (c) and for CTR/DIAM (d). The ratio has been performed both in static and in shaken conditions and is reported in the Y axis of (c,d). If the mechanical stimulus would provide a specific increase of cell lysis, this would result in systematic differences in the shaken vs. static trends. On contrary, the data show that mechanical stimulus always determines an increase in cell lysis (a vs. b) but this increasing is not mechanical stimulus-dependent as the two trends in the presence or absence of mechanical stimulus are equivalent (c,d).