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. 2022 Sep 5;23(17):10161. doi: 10.3390/ijms231710161

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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TRAF4 interacts with SETDB1. (A) Whole-cell extracts (WCE) of U87-MG and LN-229 cells were collected and subjected to immunoprecipitation assays (AKT1 for IP) and immunoblotting. The interaction of AKT1 with TRAF4 and SETDB1 in glioblastoma cells was examined. (B) Protein–protein interaction (PPI) analysis in the online tumor databases GeneMANIA. (C) 293FT cells infected with Flag-TRAF4 and MYC-SETDB1 were immunoprecipitated with anti-Flag antibody/anti-MYC antibody, followed by immunoblotting. The interaction of exogenously expressed Flag-TRAF4 and MYC-SETDB1 in 293FT was examined. (D,E) Whole-cell extracts (WCE) of (D) U87-MG and (E) LN-229 cells were collected and subjected to immunoprecipitation assays (TRAF4/SETDB1 for IP) and immunoblotting. The interaction of TRAF4 and SETDB1 in glioblastoma cells was examined.