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. 2022 Sep 5;23(17):10161. doi: 10.3390/ijms231710161

Figure 7.

Figure 7

Overexpression of SETDB1 in TRAF4 knockdown glioblastoma cells partially restores cell proliferation and AKT pathway activation. (A) The viability of TRAF4 knockdown glioblastoma cells was examined by MTT assay following SETDB1 overexpression. (B) AKT pathway activation-related protein expression in TRAF4 knockdown glioblastoma cells were detected following SETDB1 overexpression. (C) The proliferation of TRAF4 knockdown glioblastoma cells was examined by EdU assay following SETDB1 overexpression. Scale bars = 20 µm. (D) The clone-forming ability of TRAF4 knockdown glioblastoma cells detected by colony formation assay following SETDB1 overexpression. Scale bars = 5 mm. All data represent the mean ± SD from at least three independent experiments. Student’s t test was performed to analyze significance; * p < 0.05, ** p < 0.01.