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. 2022 Aug 31;23(17):9887. doi: 10.3390/ijms23179887

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phenotypic analysis of TRV:GhVIR plants. (A) Expression analysis of the second leaf of 2-leaf stage TRV:GhVIR plants and TRV:00 plants. (B) mRNA m⁶A level analysis of the second leaf of 2-leaf stage TRV:GhVIR plants and TRV:00 plants. Up-penal: mRNA m⁶A/A ratio (%) of the second leaf of 2-leaf stage TRV:GhVIR plants and TRV:00 plants by m⁶A RNA methylation quantification kit. Down-penal: dot blot analysis mRNA m⁶A level of the second leaf of 2-leaf stage TRV:GhVIR plants and TRV:00 plants. Error bars indicate SD of three biological replicates (** p < 0.01) for (A,B). (C) Whole plant phenotype comparison of 3–4 leaf stage TRV:GhVIR plants with TRV:00 plants (negative control group). (D) Second leaf phenotype comparison of 3–4 leaf stage TRV:GhVIR plants with TRV:00 plants.