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. 2022 Aug 31;23(17):9887. doi: 10.3390/ijms23179887

Figure 5.

Figure 5

Knock-down GhVIRs affect leaf morphogenesis during cotton leaf proliferation and expansion developmental stage. (A–D) Longitudinal sections of paraffin-embedded leaf were stained with toluidine blue (A–D) to show cell morphology of leaf vein and leaf blade. (A,B) longitudinal sections of leaf vein and leaf blade of TRV:00 plants, respectively. (C,D) longitudinal sections of leaf vein and leaf blade of TRV:GhVIR plants, respectively. (E,F) Super depth of field microscope pictures of the abaxial epidermal cells of the second leaf of TRV:00 and TRV:GhTRV plants. Bar = 20 μm. (G–I) The leaf area, total cell number, and cell size of the second leaf of TRV:00 and TRV:GhTRV plants. Error bars indicate SD of three biological replicates (** p < 0.01).