Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Principle of the PE system. dCas9 binds the target DNA under the guidance of pegRNA and nicks the PAM-containing strand. The hybridization of the exposed 3′ end to the PBS primes the reverse transcription of the nicked DNA strand for the desired edit. Given the preference for the 3′ flap, the 5′ flap cleavages and DNA repair installs the desired edit.
