Table 2.

Prediction of PE size with various Cas enzymes.

Ca9 Variants & Others	Cas Size (kb)	Predicted PE Size (kb)	AAV Delivery	Reference
SpCas9	4.2	6.2		[10]
St1Cas9	3.3	5.3		[23]
SaCas9	3.2	5.2	√	[6]
SauriCas9	3.1	5.3		[18]
NmeCas9	3.2	5.3		[16]
CjCas9	3.0	5.0	√	[17]
CdCas9	3.3	5.3		[19]
CasФ	2.1~2.4	4.1~4.4	√	[20]
Cas12f	1.2~1.8	3.2~3.8	√	[21]
CasX	<3.0	<5.0	√	[22]

The prediction is based on the size of Cas9 variants and orthologs, the common RT and pegRNA in Anzalone et al. [8].