TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| E. faecalis V583 | 23 | |
| B. subtilis | ||
| 168A | trpC2 | Laboratory stock |
| LUW20 | trpC2 ΔcydABCD::tet | 28 |
| LUH14 | trpC2 ΔqoxABCD::kan | Δqox→168Ab |
| Δqox | trpC2 ΔqoxABCD::kan | 26 |
| LUW173 | trpC2 ΔqoxABCD::kan ΔcydABCD::tet pCYD23 | This work |
| LUW174 | trpC2 ΔqoxABCD::kan ΔcydABCD::tet pLUF04 | This work |
| Plasmids | ||
| pHPSK | Cmr Emr | 12 |
| pCYD23 | B. subtilis cydABCD in pHP13 | 28 |
| pCYD26 | B. subtilis cyd promoter in pHPSK | This work |
| pLUF04 | E. faecalis cydABCD in pCYD26 | This work |
| pDG148 | Kmr Emr | 25 |
a
Cmr, Emr, and Kmr indicates resistance to chloramphenicol, erythromycin, and kanamycin, respectively.
b
An arrow indicates transformation and point from donor to recipient.