TABLE 2.

Functional complementation of B. subtilis cytochrome bd-deficient mutants

Recipient straina	No. of transformants (Kmr)bc	% of transformants which were also Tetrc
LUW20/pCYD23	108	99
LUW20/pLUF04	130	98
LUW20/pCYD26	3	0

^aB. subtilis LUW20, carrying a ΔcydABCD::tet mutation in the chromosome and one of three plasmids, was transformed with chromosomal DNA from LUH14, carrying a ΔqoxABCD::kan mutation. All strains showed a similar degree of competence as tested by transformation with plasmid pDG148. 

^bTransformants were selected on plates containing kanamycin and chloramphenicol and then restreaked on plates containing tetracycline. 

^cKm^r and Tet^r indicate resistance to kanamycin and tetracycline, respectively.