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. 2022 Sep 5;23(17):10199. doi: 10.3390/ijms231710199

Figure 5.

Figure 5

Tubular deletion of IKKβ affects proliferation, tissue regeneration and fibrosis. (a) assessment of Ki67-positive proliferating cells in control and IKKβ∆Tub at 2 days and 14 days after I/R injury showing the semi-quantitative evaluation and representative images. Scale bar = 50 µm. Arithmetic means ± SEM of n = 4–12 per group; (b,c) heatmaps of significantly altered mRNA for cell proliferation, apoptosis and fibrosis at 2 days (b) and at 14 days (c) after I/R injury. Filter criteria were DESeq p-values < 0.05; fold change > 1.5. All heatmaps show samples organized in columns and genes in rows, expression intensities are color-coded. (d,e) assessment of MMP9 (d) and Col3a1 (e) showing densitometric analysis of respective Western blots and representing images for each strain and time point. Scale bar = 50 µm. Arithmetic means ± SEM of n = 3–9 per group. The green stippled circle indicates mRNA of I/R groups used for relative expression in heatmaps by comparing only control with IKKβ∆Tub at 2 days (c) and 14 days (d) after I/R injury. For (a,d,e) * p < 0.05, ** p < 0.01, Mann–Whitney–U test (if n < 4 Lord test).