Figure 3.

Concentration-dependent effects of the selected compounds on the viability of breast cancer cells. (A─C) Effects of A1, A6, and SLC-0111 on the viability of MDA-MB-231 cells. (D─F) Effects of A6, A15, and SLC-0111 on the viability of MCF-7 cells. (G─I) Effects of A6, A15, and SLC-0111 on the viability of SK-BR-3 cells. Cells were treated with the indicated compounds at 1, 3, 10, 30, 100, and 300 μM for 48 h under CoCl₂-induced hypoxic condition. Control cells were treated with the media containing 0.6% DMSO instead. Cell viability was determined by MTT assay as described in the Materials and Methods. The viability of vehicle (DMSO)-treated control cells was defined as 100%, and the cell viability was expressed as a percentage of the control measured in the vehicle-treated cells in each cell line. Data are displayed as mean ± SEM from three independent experiments. IC₅₀ values were calculated using GraphPad Prism, as described in the Materials and Methods.