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. 2022 Aug 30;23(17):9833. doi: 10.3390/ijms23179833

Figure 4.

Figure 4

Phosphorylation of human LAIR1 by citrullinated collagen. Jurkat cells that overexpress the human LAIR1 (hLAIR1) were stimulated with PBS, collagen (200 µg/mL), or citrullinated collagen (200 µg/mL) for 5 min. Human LAIR1 was immunoprecipitated from 500 µg of total proteins by anti-human LAIR1 antibody (α-hLAIR1 (NKTA255), Thermo Fisher) using protein A/G bead. Phosphorylation of human LAIR1 was examined with the anti-phosphotyrosine antibody (α -pTyr 4G10, Millipore Human LAIR1 was used as control. The left panel represents type I collagen, and the right panel represents type II collagen. Data are representative of three separate analyses.