Figure 2.

DAPA administration prevents the Dox-inhibited Nrf2 nuclear translocation and expression of HO-1 and NQO1. Western blot images (A), densitometric analysis of nuclear fraction (B), and cytosolic fraction (C) of Nrf2 in Dox-treated H9c2 cells were shown. Representative Western blot images (D), densitometric analysis of HO-1 (E) and NQO1 (F) in Dox-stimulated cells with or without DAPA treatment were revealed. Expression levels of HO-1 and NQO1 gene in Dox-treated cells with or without DAPA treatment were checked (G). LY294002 (a PI3K inhibitor) and si-Nrf2 were used in some experiments. Results are shown as means ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA with a Bonferroni post hoc test. [* p < 0.05 compared to the control group; # p < 0.05 compared to the Dox-only group; & p < 0.05 compared to Dox + DAPA group].