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. 2022 Aug 26;23(17):9682. doi: 10.3390/ijms23179682

Figure 5.

Figure 5

USP48 silencing enhances NF-κB activity. (A) hTERT-RPE1 cells were transfected with scControl or siUSP48, in combination with a 3-kB-luciferase reporter plasmid (Igk-BLuc). Twenty-four hours after transfection, cells were stimulated for 4 h with TNFα and then lysed to measure NF-κB activity. n = 9 from three independent experiments conducted in triplicate. Data are represented for each experiment as normalized mean ± S.E. Statistical analysis by two-way ANOVA: * p-value ≥ 0.05; ** p-value ≥ 0.01; **** p-value ≥ 0.0001. (B) USP48 expression was efficiently silenced by siUSP48 (representative western blot). Tubulin was used as a loading control.