Figure 2.
Immunoblot analysis of Cdr1p localization in DRMs fractions from C. albicans. (A) Schematic representation of used Optipreptm gradient. (B) Wild-type CAF2-1 and ergosterol-deficient erg11∆/∆ strains were cultured for 8, 14, and 24 h in a YPD medium. Whole-cell extracts were obtained by glass-beads cell disruption, as described in Materials and Methods. Samples were subjected to Optipreptm gradient (fractions with 0%, 5%, 30% and 40%), followed by ultracentrifugation (35,000 rpm, 17 h). Collected fractions were applied on SDS-PAGE and analyzed by western blot with anti-Cdr1 and anti-GFP antibodies.