Figure 7.

Leucocyte infiltration in obstructed cftr^−/− mice treated with vehicle. (a) Representative images of MPO-stained cells (indicated by white arrows) in the jejunum, ileum, proximal colon, and mid-distal colon in cftr^+/+ and cftr^−/− mice treated with either the vehicle or tenapanor (n = 5/genotype/group/intestinal segment). (b) Counting of MPO-positive cells showed obvious leucocyte infiltration in the ileum and proximal colon of obstructed cftr^−/− mice treated with vehicle. (c) Ly6g mRNA expression further confirmed this observation. Each dot/triangle represents one mouse. Veh—vehicle, Ten—tenapanor, UO—unobstructed, O—obstructed. Data are represented as means ± SEM. # is used to represent the statistical analysis performed between the two different genotypes and * is used to represent the statistical analysis performed between the vehicle and tenapanor groups or between unobstructed and obstructed vehicle groups. # and *, p < 0.05.