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. 2022 Aug 30;23(17):9848. doi: 10.3390/ijms23179848

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of LEE on CXCR7/4 and several EMT markers in presence of CXCL12 stimulation. The cells were stimulated with CXCL12 (10 ng/mL) and treated with LEE (1 μM) for 24 h. Whole cell lysates were analyzed by Western blot analysis. The protein expression level of (A) CXCR7/4, (B) MnSOD, and (C,D) several EMT markers (fibronectin, vimentin, MMP-2/9, N-cadherin, twist, snail, occludin, E-cadherin) were probed using specific antibodies. (E) The supernatants were obtained and concentrated, and then activity of MMP-9/2 was evaluated by zymography. All the experiments were individually repeated at least thrice.