Figure 8.
Effect of Cur, FA, or a combination of both on membrane phospholipid peroxidation level in Aβ1–42-stimulated SH-SY5Y cells. The levels of membrane phospholipid peroxidation in Aβ1–42-stimulated SH-SY5Y cells was evaluated using DPPP. The levels of membrane phospholipid peroxidation in SH-SY5Y cells exposed to 5 µM Aβ1–42 and treated with Aβ1–42 + 1 µM Cur, Aβ1–42 + 10 µM FA or Aβ1–42 + Cur + FA. In the absence of 5 μM Aβ1–42, the membrane phospholipid peroxidation levels of control, 1 μM Cur, 10 μM FA and Cur + FA-treated cells were 20.58 ± 2.41, 21.97 ± 2.18, 18.31 ± 1.54 and 17.77 ± 1.93 fluorescence intensity × 106/μg protein (no significant difference, n = 6, Tukey). +: inclusion of 5 μM Aβ1–– 42, 1 μM Cur, 10 μM FA, respectively, −: non-inclusion. The p-values in ANOVA were <0.001. Each value expresses the mean + S.E.M. of at least 3 independent experiments. #, p < 0.05; ##, p < 0.01 for Aβ1–42 exposed cells versus the other treated cells (n = 6, Tukey); *, p < 0.001; for Aβ1–42 + Cur + FA-treated cells versus Cur-treated cells (n = 6, Tukey).