Figure 10.

Top-50 DEGs in altered eyes of old (two-year-old) male myoc transgenic zebrafish. (A) Down- and (B) up-regulated genes identified by high-throughput RNA sequencing with significant differences coinciding in the comparison with the two wild-type ocular transcriptomes (Tg/+ OA vs. +/+1 and Tg/+ OA vs. +/+2). Confirmation by qRT-PCR of differential gene expression of selected (C) down- and (D) up-regulated genes. Aliquots of RNA preparations used for transcriptomic analyses were used as templates in qRT-PCR. Values represent the average of six experimental replicas. **: p < 0.01; ***: p < 0.001, Student’s t-test.