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. 2022 Sep 2;23(17):10013. doi: 10.3390/ijms231710013

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Detection of PxMETTL3 and PxMETTL14 mutants and changes in m⁶A levels. Schematic diagrams of two sgRNA target sites are presented (A,B). The sgRNA-targeting and PAM sequences are highlighted in red and blue, respectively. PxMETTL3 and PxMETTL14 mutant sequences were confirmed by cloning and sequencing. (C) m⁶A level of total RNA. WT-AD, wildtype individuals of the artificial diet strain; ΔPxMETTL3-2, ΔPxMETTL3-2 mutant strain generated from WT-AD; ΔPxMETTL14-14, ΔPxMETTL14-14 mutant strain generated from WT-AD. The “ns” indicates no significant difference (p > 0.05) and ** means significant level at p < 0.01.