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. 2022 Jul 29;42(9):848–867. doi: 10.1002/cac2.12341

FIGURE 2.

FIGURE 2

PTPRO represses CRC cell growth and liver metastasis. (A) PTPRO expression levels in six CRC cell lines and NCM460 determined by Western blotting and qRT‐PCR. (B) Western blotting and qRT‐PCR verify the shRNA‐mediated deletion of PTPRO in SW480 and LoVo cells. (C) Western blotting and qRT‐PCR verify the PTPRO expression in HCT116 and SW620 cells with transfection of the control PCDH retrovirus or the PCDH‐PTPRO retrovirus. (D) Migration assay of SW480 and LoVo cells with PTPRO‐NC or PTPRO‐KD. (E) Migration assay of HCT116 and SW620 cells with PTPRO‐Vector or overexpression. (F‐H) Subcutaneous xenograft tumor images and tumor growth of LoVo cells with PTPRO‐NC and PTPRO‐KD in nude mice. (I‐J) The gross images (I) and number (J) of liver metastasis induced by LoVo cells with PTPRO‐NC and PTPRO‐KD in nude mice. *P < 0.05. PTPRO: protein tyrosine phosphatase receptor type O; CRC: colorectal cancer; qRT‐PCR: quantitative real‐time polymerase chain reaction; NC, negative control; KD: knockdown