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. 2022 Aug 29;119(36):e2203452119. doi: 10.1073/pnas.2203452119

Fig. 1.

Fig. 1.

CTCF loss of heterozygosity promotes invasiveness and unorganized growth in distinct breast epithelial models. (A) Western blot showing low levels of CTCF, similar to the CTCF+/− MCF10A, in the PDX cells. Loading CTL: actin. (B) Western blot of ectopic HA-CTCF expression in PDX cell lines. Loading CTLs: actin and tubulin. (C) Decrease in relative invasiveness of HA-CTCF PDXs to their respective GFP CTLs (mean ± standard error of mean (SEM]). P = 0.0031, 0.0084, and 0.0015 for PDX 1, 2, and 3, respectively. (D) Western blot of low CTCF levels in CTCF+/− compared to CTL MCF10A. Quantification of relative CTCF band intensity in CTCF+/− to CTL. Loading CTL: actin. Bar chart of the increased relative invasiveness of CTCF+/− to CTL (mean ± SEM). P = 0.0066 and P < 0.0001 for CTCF+/− 1 and 2, respectively. (E) Decrease in relative invasiveness of CTCF+/− MCF10A with HA-CTCF addback to their respective GFP CTLs (mean ± SEM). P = 0.0013 for both CTCF+/− 1 and 2. P value indicators, *P = 0.05, **P = 0.01, ***P = 0.001, ****P = 0.0001. See also SI Appendix, Fig. S1.