Table 4.
Analytical method validation.
| Analyte | Standard Concentration (nmol L−1) | Recovery (%) | LOD (nmol L−1) |
LOQ (nmol L−1) | Linearity Range (nmol L−1) |
Equation (y = a + bx) a ± sa b ± sb R2 |
|---|---|---|---|---|---|---|
| 18:1 LPE | 6.25 | 108 ± 14 | 0.548 | 1.83 | 1.83–26.30 | 0.0019 ± 0.0008 |
| 9.38 | 109 ± 15 | 0.0027 ± 0.000063 | ||||
| 12.5 | 104 ± 17 | 0.998 | ||||
| 18:0 LPC | 50 | 153 ± 15 | 4.185 | 13.95 | 13.95–209.38 | 0.012 ± 0.024 |
| 75 | 147 ± 15 | 0.0072 ± 0.00022 | ||||
| 100 | 134 ± 21 | 0.997 | ||||
| 16:1 SM (d18:1/16:1) | 50 | 101 ± 11 | 2.857 | 9.52 | 9.52–208.11 | 0.0774 ± 0.021 |
| 75 | 101 ± 11 | 0.0064 ± 0.00019 | ||||
| 100 | 96 ± 16 | 0.997 | ||||
| 16:0 SM (d18:1/16:0) | 12.5 | 108 ± 58 | 1.240 | 4.13 | 4.13–52.51 | −0.0041 ± 0.0063 |
| 18.75 | 102 ± 6 | 0.012 ± 0.00024 | ||||
| 25 | 82 ± 5 | 0.999 | ||||
| 18:0 SM (d18:1/d18:0) | 3.13 | 0.289 | 0.96 | 0.96–13.23 | 0.0014 ± 0.0011 | |
| 4.69 | 100 ± 26 | 0.0047 ± 0.00017 | ||||
| 6.25 | 119 ± 59 | 0.996 | ||||
| 18:1 (9-Cis) PE (DOPE) | 0.78 | 0.069 | 0.23 | 0.23–3.30 | 0.00019 ± 0.00015 | |
| 1.17 | 103 ± 65 | 0.0024 ± 0.000089 | ||||
| 1.56 | 62 ± 62 | 0.996 | ||||
| 24:0 SM | 6.25 | 0.306 | 1.02 | 1.02–26.02 | 0.24 ± 0.03 | |
| 9.38 | 0.044 ± 0.003 | |||||
| 12.50 | 0.990 |