Figure 7.

Light explanation microscopy for histochemistry apoptotic cells (A) Mouse liver section from the first group displaying no TUNEL-positive apoptotic cells in the healthy tissues of hepatocytes around the central hepatic vein (CV) (TUNEL, 400×). (B) Mouse liver section from the second group showing the normal distribution of normal hepatocytes in the hepatic cord found around the central vein (CV) (TUNEL, 400×). (C) The mouse liver section from the third group that detected the distribution of apoptotic cells (red arrows) was displayed in the liver tissues after the mice were exposed to Fe₂O₃-nanoparticles; the inflammatory cells were found around the central vein (CV), and vacuolation (asterisks) was observed (TUNEL, 400×). (D) Mouse liver section from the fourth group showing a cure in the apoptotic cells (red arrows) within the hepatocytes which were located around the hepatic central veins (CV) and a mild appearance of vacuolation (asterisk) (TUNEL, 400×); this shows the usefulness of using PPE in the improvement of toxicity, which was induced in the morphology of the liver tissues of mice exposed to Fe₂O₃-NPs.