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. 2022 Aug 30;27(17):5574. doi: 10.3390/molecules27175574

Figure 1.

Figure 1

SDS-PAGE analysis of CD47-His after the Ni column showed a molecular weight of ~34 kDa (left figure). Based on the result, fractions of flow through 4–14 were pooled together and concentrated by an ultrafiltration tube with a molecular cut-off value of 10 kDa (middle figure). The SDS-PAGE analysis of final CD47-His showed a clean band with a molecular weight of ~34 kDa (right figure). The theoretical molecular weight of CD47-His is 14.8 kDa. The SDS-PAGE re-sults show that the actual molecular weight is ~34 kDa due to the severe glycosylation modifica-tion of CD47.