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IMM01-Fab and CD47-His were mixed in molar ratio 1:1 and incubated at 4 °C overnight. The size-exclusion chromatography (SEC) method was used for IMM01-Fab/CD47 complex purification. Superdex200 column was used to increase purity of IMM01-Fab/CD47 complex. Fractions 23–26 were concentrated for crystallization.
