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. 2022 Aug 29;27(17):5545. doi: 10.3390/molecules27175545

Figure 1.

Figure 1

Resveratrol treatment improves stability of ΔΨm, ATP content, SOD content and MDA content in neonatal rat cardiomyocytes undergoing H/R, while this effect was abrogated by EX527. The cardiomyocytes were pretreated with resveratrol at doses of 5 or 20 µM and resveratrol combined with Ex527 (1 µM) treatment exposed to hypoxia for 12 h and reoxygenation for 12 h. (A) Mitochondrial membrane potential (ΔΨm) was determined by JC-1 dye, observed by inverted fluorescence microscope. Red aggregate emission profile represents the JC-1 probe accumulates in healthy mitochondria, whereas green emission represents mitochondrial injury and loss of membrane potential. The graph represents the ratio of red/green fluorescence. (B) ATP content was measured by inverted fluorescence microscope and microplate reader. (C) SOD content was measured by SOD detection kit-WST. (D) MDA content was measured by MDA test kit. Figures are representative images of three different samples. Results are expressed as mean ± S.D. * p < 0.05, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. H/R group; ∆ p < 0.05, ∆∆∆ p < 0.001 vs. Res-20 group.