Resveratrol treatment regulates mitochondrial function, fission–fusion and biosynthesis in neonatal rat cardiomyocytes after H/R, while this effect was abrogated by EX527. (A) Representative picture of mitochondria probed with MTG and MTR through laser-scanning confocal microscopy. RT-PCR was performed with cardiomyocytes lysate for relative expression of (B) Mfn1, Mfn2 and Opa1, (C) Drp1 and Fis1, (D) PGC-1α. Figures are representative images of three different samples. Results are expressed as mean ± S.D. * p < 0.05, *** p < 0.001 vs. control group; #
p < 0.05, ###
p < 0.005 vs. H/R group; ∆
p < 0.05 vs. Res-20 group.